Fig. 2.

Histological analysis and cell recruitment after implantation of the dECM material. (A) Representative images of dECM stained with HE, Masson, and EVG staining on days 3, 7, 14, and 28 after implantation in the mouse muscle traumatic model. Fibroblasts are indicated by green arrows, neutrophils by yellow arrows, monocytes by red arrows, capillaries by blue arrows, multinucleated giant cells by white arrows, and fibrous connective tissue by black arrows. The dECM boundaries are framed with black wire. (B, C) Representative FACS plots (B) and quantitative analysis (C) of the proportion of CD45⁺ cells between the dECM and sham groups on days 3, 7, 14, and 28 after implantation. (D, E) Proportion of Tregs in the dECM and sham groups was determined on days 3, 7, 14, and 28 after implantation via flow cytometry (D) and quantitative analysis (E). (F, G) Representative images (F) and statistical plots (G) of Foxp3⁺ cells in the dECM material, detected via immunofluorescence on days 3, 7, 14, and 28 after implantation. ns = not significant, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)