Fig. 3.

Establishment of a mouse model with reduced Treg recruitment and histological analysis of the dECM material after implantation. (A) Schematic of the protocol for establishing a mouse model with reduced Treg recruitment. (B) Flow cytometry analysis of the proportion of Tregs in the peripheral blood of mice throughout the experimental period, indicating that the proportion of Tregs remains low. (C, D) Flow cytometry analysis results of the Treg proportions in the dECM, peripheral blood, and lymph nodes on day 14 after dECM material implantation, indicating a decrease in the proportion of Tregs. (E, F) Immunofluorescence images of Foxp3⁺ cells from dECM material on day 14 after implantation show statistically decreased infiltration of Tregs into the dECM. (G) HE images of the dECM material in the anti-CD25 and control groups. Neutrophils are indicated by yellow arrows, capillaries by blue arrows, and fibroblasts by green arrows. (H) Immunofluorescence images of F4/80⁺ cells of the dECM material between the anti-CD25 and control groups. (I) Representative images of Masson and EVG of the dECM material between the anti-CD25 and control groups. (J) Statistical analysis of the collagen fiber area percentage in the Masson images. (K) Statistical analysis of the elastic fiber area percentage in EVG images. (L) Hydroxyproline was used to determine the relative collagen content of the dECM material between the anti-CD25 and control groups. (M) Relative elastin content of the dECM material between the anti-CD25 and control groups. **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)