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. 2024 Jul 9;27:101151. doi: 10.1016/j.mtbio.2024.101151

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© 2024 The Authors

This is an open access article under the CC BY-NC license (http://creativecommons.org/licenses/by-nc/4.0/).

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Fig. 4 — Following a decrease in Treg recruitment, the presence of M2 macrophages decreases. (A–C) Representative immunofluorescence images (A), and quantitative analysis of total macrophages (B) and M2 macrophages (C). (D, E) Representative immunofluorescence images (D) and quantitative analysis (E) of M1 macrophages. (F) Representative FACS plots of macrophage polarization determined via flow cytometry. (G–H) Quantitative analysis of the proportion of M2 (G) and M1 (H) macrophages detected via flow cytometry. ns = significant, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.