Fig. 5.

Treg recruitment increases after injection of a CD28-SA antibody. (A) Schematic of the protocol for establishing a mouse model with increased Treg recruitment. (B) Flow cytometry results of the Treg proportion in the peripheral blood of mice indicate a persistently high proportion of Tregs throughout the study period, relative to the control group. The results were normalized relative to those of the control group. (C, D) Representative FACS plots of Treg proportion in dECM, peripheral blood, and lymph nodes on day 14 after dECM material implantation (C), with the accompanying statistical graph (D) indicating an increase in Treg proportion. (E, F) Immunofluorescence image of Foxp3⁺ cells in dECM material on day 14 after implantation (E), with the accompanying statistical results (F) indicating an increase in Tregs infiltrating the dECM interior. **p ≤ 0.01, ****p ≤ 0.0001.