Fig. 7.

Increased Treg recruitment promotes the anti-inflammatory and pro-regenerative phenotype of macrophages. (A–C) Representative immunofluorescence images (A), and quantitative analysis of total macrophages (B) and M2 macrophages (C). (D, E) Representative immunofluorescence images of M1 macrophages (D) and quantification analysis (E). The proportion of positive cells was counted by scanning and counting the entire dECM material. Statistical analysis was performed using T-test (n = 5) and the data are presented as mean ± SD. (F) Representative FACS plots of macrophage polarization. (G) Flow cytometry statistical analysis revealing an increased proportion of M2 macrophages. (H) Flow cytometry statistical analysis, depicting a reduction in the proportion of M1 macrophages. (I) Volcano plot of the differentially expressed genes in F4/80⁺ macrophages selected from the dECM material. (J) Heat map of macrophage polarization and inflammation-related gene expression (red: highly expressed; blue: low expression). (K) GSEA pathway enrichment map. (L) RT-qPCR results for verifying the expression of CD163, Arg1, and TNF-α. *p ≤ 0.05, ***p ≤ 0.001, ****p ≤ 0.0001. The test result of one asterisk (*) is annotated using the P-value on the graph. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)