Fig. 8.

Treg-conditioned medium promotes M2 macrophage polarization in vitro. (A, B) M0 BMDMs were polarized with soluble dECM material, while Treg-conditioned medium was added in parallel. Representative FACS plots of macrophage polarization using flow cytometry (A) and accompanying statistical analysis results (B). (C, D) Representative plots of CD206 and iNOS protein expression in macrophages based on Western blot (C), and corresponding quantitative analysis for CD206 protein expression (D). (E, F) Relative mRNA expression levels of M2 macrophage-associated CD206 and IL10 (E) and M1 macrophage-associated CD80 and TNF-α (F) based on RT-qPCR. *p ≤ 0.05, ****p ≤ 0.0001.