Fig. 4.

PITSA Reveals Thermal Stability Regulation of the TCR Signaling Pathway.A, a comparison of the difference between the phosphorylation site and protein thermal shift (Δ Phospho-Protein TS) versus phosphorylation site abundance for the TCR signaling pathway (derived from Fig. 3B). Lines represent 2-fold change. B, a schematic of the TCR signaling pathway, with regulated phosphorylation sites from (A) indicated; colors coordinate with categories from (A). Phosphorylation site abundance and thermal shift for CBL and CBLB (C) as well as LCK (D). Values are the mean Log₂ fold change of the indicated timepoint versus 0 h of n = 2 to 4 replicates. Error bars (C and D) represent SEM.