Figure 4.

Iron content and types of iron-sulfur clusters in three types of MagRs

A: UV-Vis absorption spectra of purified MagR or IscA type proteins. Insert picture shows protein solution. B: CD spectra of purified ecIscA (black line), ceMagR (red line), and clMagR (blue line) and hsMagR (green line), representing the three types of MagR proteins, respectively. C: Comparison of EPR spectra recorded at 10 K for ecIscA, ceMagR, clMagR, and hsMagR to detect ferric iron binding in three types of MagR proteins. D–G: EPR spectra of ecIscA (D), ceMagR (E), and clMagR (F) and hsMagR (G) representing three types of MagR proteins, respectively.