Figure 4.

MP exposure disturbed sleep structure in flies

A: Schematic of D. melanogaster activity monitoring system. Sleep activity was detected by recording crossing changes in infrared beams at the tube center. B: Adult flies were raised on standard food from days 1–2, then transferred to standard food or MP mixed in food at two doses: 1×MPs and 10×MPs from days 3–8. Sleep profile of total sleep with/without MP food across 8 days (D represents day). C: Quantitative analysis of changes in sleep amount, maximum episode length, mean episode length, number of episodes, and activity while awake during daytime (LP, light period) and nighttime (DP, dark period) showed that 1×MP treatment resulted in a significant increase in maximum episode length during daytime (n=31–43 per group; one-way ANOVA followed by Bonferroni’s post-hoc analysis, ^*: P<0.05).