Extended Data Fig. 5|. 7-DHC is a general suppressor of ferroptosis.

a, Cell viability of various cancer cell lines treated with indicated concentrations of RSL3 for 6–8 h after pretreatment of 7-DHC (25 μM) for 24 h. For HuH-7, PLC/PRF/5 and SK-Hep1, n=2 biological replicates and for 786-O, U2OS and HT1080, n=3 biological replicates. b, Cell viability of U2OS and RCC4 treated with indicated concentrations of Erastin for 16 h after pretreatment of 7-DHC (25 μM) for 24 h. n=2 biological replicates. c, d, Cell viability of various human cancer cell lines (human hepatocellular carcinoma cell lines HuH-7, PLC/PRF/5 and SK-Hep1; human kidney cancer cell lines Caki-1; human kidney cancer cell line 786-O; human osteosarcoma cell line U2OS; human fibrosarcoma cell line HT1080) treated with indicated concentrations of RSL3 for 8–12 h after pretreatment of AY9944 (100 nM) (c) or cariprazine (100 nM) (d) for 48 h in LPDS-supplemented medium. For Caki1, n=2 biological replicates and for the other cell lines, n=3 biological replicates. For a-d, data are representative of at least two independent experiments. Data are shown as the mean ± s.d. when n=3 biological replicates (a, c, d).