Extended Data Fig. 10 |. Cariprazine protects IRI in vivo.

a, Levels of 7-DHC in serum of mice intraperitoneal injected of 7-DHC after indicated time. n=3 in each group. b, Levels of 7-DHC in serum of mice kidney IRI model with pretreatment of AY9944. n=7 in each group. c, Workflow of mice kidney IRI model with pretreatment of indicated dose of cariprazine via intraperitoneal injection once daily for four times before IRI. d, e, Levels of 7-DHC in serum (d) and kidneys (e) of mice kidney IRI model with pretreatment of indicated dose of cariprazine. f, Body weight of IRI model mice with indicated treatments over time. g, h, The concentration of BUN (g) and SCr (h) of mice IRI model. i, Workflow of IRI model with pretreatment of cariprazine at the dose of 5mg/kg via intraperitoneal injection once daily for four times before IRI. j, Body weight of IRI model mice with indicated treatment over time. k, l, The concentration of BUN (k) and SCr (l) of mice kidney IRI model with indicated treatment over time. m, Levels of 7-DHC in kidneys of mice IRI model with pretreatment of cariprazine. n, o, Representative PAS staining (n) and quantification of renal damage (o) from mice IRI model. Scale bar, 100 μm. For d-h, n=3 in each group, and for i-o, n=7 in each group. For (a and d-o), data plotted are mean ± s.e.m. from one experiment. For b, data are representative of two independent experiments and data plotted are mean ± s.e.m. Statistical analysis was performed using one-way ANOVA (a, d-h) and two-way ANOVA (b, j-m, o), *P < 0.05, **P < 0.01 ***P < 0.001, ****P < 0.0001, ns, not significant. p, Working model of 7-DHC. 7-dehydrocholesterol (7-DHC) protects cells from lipid peroxidation on both plasma membrane and mitochondria via diverting the peroxidation pathway from phospholipids, thus mitigating ferroptosis. PLOOH, phospholipid hydroperoxide; PLOO·, phospholipid hydroperoxyl radical. Image created with BioRender.