Fig. 5.

The mossy fibers express GAD₆₇. A1) Panoramic view of the mossy fiber projection showing, on the left hand, two GFP⁺ GCs (arrow) and ${\text{GAD}}_{67}^{+}$ mossy fibers. The area shown at high magnification in A2 is signaled. A2) Confocal image of an axon of a GFP⁺ granule cell is shown along the stratum lucidum of CA3. B) Confocal images (50 optical slices of 0.5 μm) of mossy fiber giant boutons expressing GAD₆₇ at 15 dpi. C) Mossy fiber boutons of GFP⁺ GCs at 30 dpi, did express GAD₆₇ enzyme. D) Three-dimensional reconstructions through the Z-axis of GFP⁺ boutons (fifty optical slices at 0.5 μm intervals) were used to verify the co-localization of GFP and GAD₆₇. Orthogonal projections are shown on the right most panel.