FIG. 7.

Effect of the Golgi apparatus-disrupting agent BFA on the formation of KUN-induced cytoplasmic foci as detected by IF. Infected cells were incubated with (a to f) or without (g and h) BFA (10 μg/ml) at different times p.i., as indicated, and the relative distributions of anti-dsRNA (a, c, e, and g) and anti-NS3 (b, d, f, and h) antibodies were analyzed after acetone fixation and subsequent dual labelling with Texas red- and FITC-conjugated antibodies, respectively.