FIG. 1.

Schematic representation of the full-length MV plasmids used for virus rescue. The solid lines indicate the positions of the intergenic trinucleotide spacers. The positions of the T7 promoter, hepatitis delta ribozyme (δ), and T7 terminator (TΦ) are indicated (not to scale). Open reading frames encoding the virus structural genes are shaded, and their flanking untranslated regions are represented as open boxes. The genome length of each virus is given in nucleotides (nt). (A) Structure of p(+)MV encoding the MV Edmonston B strain antisense genome (Edtag). The sizes of the 3′ and 5′ untranslated regions of the N gene are indicated. (B) Structure of pMeGFPNV encoding the MV Edmonston B strain antisense genome with an ATU which is composed of the open reading frame of EGFP flanked by sequences based on the 3′ and 5′ untranslated regions of the N gene. A total of 852 additional base pairs are present in pMeGFPNV. The sizes of the untranslated regions are indicated below and above the EGFP and N gene segments respectively.