In vivo half-life and safety of fluorous proteins.
(a) Time-dependent activity of β-Gal in plasma delivered intravenously
in either saline (β-GalSaline, blue) or extracted
PFOc (β-GalExt. PFOc, green). Data shown as
average ± s.d. of n = 5 technical replicates.
Statistical significance calculated with Student’s t test, with n.s. = not significant and *p < 0.05. (b) Serum toxicology results from C57BL/6J mice 24 h
after injection of saline (control), β-GalSaline,
or β-GalExt. PFOc. Data shown as box and whisker
plot ± s.d. of n = 5 technical replicates. Statistical
significance calculated using Student’s t test
and represented as * p < 0.05; data subsets with p > 0.05 represented nonsignificant changes. (c) Representative
histology images of lung, kidney, liver, and spleen tissue section
from C57BL/6J mice 24 h after administration of saline (control),
β-GalSaline, or β-GalExt. PFOc. Each imaging group consisted of n = 4 mice, with
detailed tissue section evaluation conducted by an unblinded board-certified
veterinary anatomic pathologist. Scale bar: 100 μm.