. 2024 Jul 17;72(30):16749–16760. doi: 10.1021/acs.jafc.4c03264

Table 1. Fatty Acid Composition of Microalgae Lipid Extracts Determined by GC–MS Using the Traditional Folch Method and Ultrasound-Assisted Extraction (UAE)^a.

	% fatty acids
	M. gaditana		T. lutea		P. tricornutum		P. cruentum
fatty acid	Folch	UAE	Folch	UAE	Folch	UAE	Folch	UAE
14:0	4.2 ± < 0.1^b	4.4 ± 0.1^a	17.6 ± 0.6^b	18.7 ± 0.4^a	8.0 ± 0.2^a	7.8 ± 0.3^a	n.d.	n.d.
15:0	n.d.	n.d.	0.8 ± 0.1^a	0.9 ± 0.1^a	n.d.	n.d.	n.d.	n.d.
16:0	20.7 ± 0.1^a	19.5 ± 0.5^b	11.6 ± 0.4^b	13.9 ± 1.1^a	24.9 ± 0.5^b	25.8 ± 0.2^a	44.3 ± 0.5^b	45.7 ± 0.4^a
16:1n-7	21.6 ± 0.2^a	22.0 ± 0.5^a	7.2 ± 0.1^a	6.9 ± 0.1^b	25.7 ± 0.2^a	25.7 ± 0.5^a	n.d.	n.d.
16:3n-3	n.d.	n.d.	n.d.	n.d.	12.7 ± 0.8^a	13.4 ± 0.3^a	n.d.	n.d.
16:4	n.d.	n.d.	n.d.	n.d.	1.5 ± 0.1^b	2.1 ± 0.1^a	n.d.	n.d.
18:0	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	0.5 ± < 0.1^a	0.4 ± < 0.1^a
18:1n-7	0.6 ± < 0.1^a	0.7 ± < 0.1^a	n.d.	n.d.	1.1 ± < 0.1^b	1.4 ± < 0.1^a	0.8 ± 0.2^a	0.9 ± < 0.1^a
18:1n-9	3.9 ± 0.2^a	3.9 ± 0.1^a	n.d.	n.d.	3.4 ± 0.1^a	3.1 ± 1.0^b	0.8 ± 0.2^a	1.0 ± 0.1^a
18:2n-6	3.6 ± 0.2^a	3.7 ± 0.1^a	3.6 ± 0.1^a	3.5 ± 0.1^a	2.3 ± < 0.1^a	2.2 ± < 0.1^a	6.4 ± 0.2^a	6.4 ± 0.1^a
18:3n-3	n.d.	n.d.	12.1 ± 0.5^a	10.8 ± 0.3^b	n.d.	n.d.	n.d.	n.d.
18:4n-3	n.d.	n.d.	32.7 ± 1.3^a	31.8 ± 1.4^a	n.d.	n.d.	n.d.	n.d.
20:2n-6	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	1.9 ± 0.1^a	1.6 ± 0.1^b
20:3n-6	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.	0.9 ± 0.1^a	0.7 ± < 0.1^b
20:4n-6	4.0 ± 0.1^a	3.2 ± 0.1^b	n.d.	n.d.	0.6 ± < 0.1^a	0.6 ± < 0.1^a	23.7 ± 0.3^a	22.7 ± < 0.1^a
20:5n-3	41.5 ± 0.4^a	42.2 ± 0.7^a	n.d.	n.d.	18.5 ± 0.8^a	16.9 ± 0.4^b	20.8 ± 0.3^a	20.8 ± 0.4^a
22:0	n.d.	n.d.	n.d.	n.d.	0.2 ± < 0.1^a	0.2 ± 0.2^a	n.d.	n.d.
22:5n-6	n.d.	n.d.	1.4 ± 0.2^a	1.4 ± 0.3^a	n.d.	n.d.	n.d.	n.d.
22:6n-3	n.d.	n.d.	13.0 ± 1.1^a	12.1 ± 0.5^a	0.9 ± 0.1^a	0.8 ± < 0.1^b	n.d.	n.d.
SFA	24.9 ± 0.1^a	24.2 ± 0.4^b	30.0 ± 0.1^b	33.4 ± 1.4^a	33.2 ± 0.6^a	33.8 ± 0.2^a	44.8 ± 0.4^a	46.0 ± 0.4^a
MUFA	26.1 ± 0.1^a	26.7 ± 0.4^a	7.2 ± 0.1^a	6.9 ± 0.1^b	30.2 ± 0.2^a	30.2 ± 0.6^a	1.2 ± < 0.1^a	1.5 ± 0.4^a
PUFA	49.0 ± 0.1^a	49.1 ± 0.7^a	62.8 ± 0.2^a	59.7 ± 1.4^b	36.6 ± 0.8^a	36.1 ± 0.5^a	53.6 ± 0.4^a	52.2 ± 0.5^b
n-3	41.5	42.2	57.9	54.8	32.1	31.1	20.8	20.8
n-6	10.6	10.7	4.9	4.9	2.9	2.8	32.8	31.3
n-6/n-3 ratio	0.3	0.3	0.1	0.1	0.1	0.1	1.6	1.5

Results are expressed as a percentage of the total content (relative content). Data is shown as mean ± SD (n = 3). Different letters indicate statistically significant differences between extraction methods for each species at p < 0.05 (one-way ANOVA with a post hoc Tukey test a,b). SFA, saturated fatty acids; MUFA, monounsaturated fatty acids; PUFA, polyunsaturated fatty acids; n.d., not detected.

Table 1. Fatty Acid Composition of Microalgae Lipid Extracts Determined by GC–MS Using the Traditional Folch Method and Ultrasound-Assisted Extraction (UAE)a.

Table 1. Fatty Acid Composition of Microalgae Lipid Extracts Determined by GC–MS Using the Traditional Folch Method and Ultrasound-Assisted Extraction (UAE)^a.