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Primer Sequences of Each Gene Detected in RT-PCR
| Gene | Sequence | |
|---|---|---|
| ACTB | Forward | 5′-GCCGACAGGATGCAGAAGGAGATCA-3′ |
| Reverse | 5′-AAGCATTTGCGGTGGACGATGGA-3′ | |
| IL-1β | Forward | ATGATGGCTTATTACAGTGGCAA |
| Reverse | GTCGGAGATTCGTAGCTGGA | |
| IL-6 | Forward | ACTCACCTCTTCAGAACGAATTG |
| Reverse | CCATCTTTGGAAGGTTCAGGTTG | |
| IFN-α | Forward | TCGGTAACTGACTTGAATGTCCA |
| Reverse | TCGCTTCCCTGTTTTAGCTGC | |
| TNF-α | Forward | GAGGCCAAGCCCTGGTATG |
| Reverse | CGGGCCGATTGATCTCAGC | |
