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. 2024 Jun 30;73(3):415–426. doi: 10.33549/physiolres.935291

Fig. 3.

Fig. 3

MiR-539-3p attenuated childhood-onset OA chondrocyte injury in vitro. (A) The morphology of chondrocytes and results of toluidine blue staining (blue, ×200). Childhood-onset OA chondrocytes were transfected with miR-539-3p mimics, NC mimics, miR-539-3p inhibitor or inhibitor NC for 48 h. (B) The expression of miR-539-3p was detected by quantitative real time PCR in transfected chondrocytes. (C) Cell viability was analyzed in transfected chondrocytes by CCK-8 assay. (D) The concentration of IL-6 and TNF-α in the supernatants of transfected chondrocytes was determined by ELISA assay. (E) Cell apoptosis was detected in transfected chondrocytes by flow cytometry. (F) The protein levels of RUNX2, caspase-3, Bcl-2, COL2A1 and MMP-13 were detected in transfected chondrocytes by western blot analysis. All data were presented as the mean ± SD of three independent experiments. * p<0.05, ** p<0.01, *** p<0.001, compared with mimics NC; # p<0.05, ## p<0.01, ### p<0.001, compared with inhibitor NC.