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. 2024 Jun 30;73(3):415–426. doi: 10.33549/physiolres.935291

Fig. 5.

Fig. 5

MiR-539-3p inhibited childhood-onset OA chondrocyte injury in vitro through downregulating RUNX2. Childhood-onset OA chondrocytes were co-transfected with miR-539-3p mimics and pcDNA4.0-RUNX2, or miR-539-3p inhibitor and si-RUNX2 for 48 h. (A) Cell viability was analyzed in transfected chondrocytes by CCK-8 assay. (B–C) The concentration of IL-6 and TNF-α in the supernatants of transfected chondrocytes was determined by ELISA assay. (D) Cell apoptosis was detected in transfected chondrocytes by flow cytometry. (E) The protein levels of RUNX2, caspase-3, Bcl-2, COL2A1 and MMP-13 were detected in transfected chondrocytes by western blot analysis. All data were presented as the mean ± SD of three independent experiments. * p<0.05, ** p<0.01, *** p<0.001, compared with mimics + pcDNA4.0; # p<0.05, ## p<0.01, ### p<0.001, compared with inhibitor + si-NC.