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. 2023 Dec 7;27(4):1125–1136. doi: 10.1007/s10123-023-00463-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Genetic clusters identified among invasive Portuguese isolates of N. meningitidis serogroup B during the period 2012 to 2020. The genetic diversity among isolates was evaluated by a gene-by-gene analysis using the cgMLST schema v2, with 1422 N. meningitidis core-loci. The Minimum spanning tree was generated with the MSTreeV2 method of GrapeTree and was based on allelic diversity found among 251 validated MenB isolates (Mixão et al. 2023). For a better visualization of the identified gene clusters, nodes were collapsed. Filled small circles (whose size is proportional to the number of isolates it represents) represent unique allelic profiles and were colored by year of isolation with clonal complexes represented by large, shaded circles. The numbers in grey on the connecting lines represent the allele differences between isolates