Fig. 8. Rescue of GSDME in cardiomyocytes but not in myeloid cells replicates aPD-1 therapy-induced cardiac immune cells infiltration and inflammation.

A Representative fluorescent immunohistochemistry staining of CD8 and CD4 and amplified images in whole heart section of WT, Gsdme^Stop/Stop, Gsdme^CR and Gsdme^MR mice received aPD-1 therapy. The infiltrated CD8⁺ and CD4⁺ T-cells were obviously noted in the myocardium of WT and Gsdme^CR mice, which were partially inhibited in Gsdme^Stop/Stop and Gsdme^MR mice. Scale bar, 100 μm. B Representative flow cytometry and quantitative analysis of CD4⁺ and CD8⁺ cells in heart of WT, Gsdme^Stop/Stop, Gsdme^CR and Gsdme^MR mice received aPD-1 therapy. C Proportions of Th1 (IFN-γ⁺), Th2 (IL-4⁺) and Th17 (IL-17A⁺) cells within CD4⁺ T-cells in hearts of WT, Gsdme^Stop/Stop, Gsdme^CR and Gsdme^MR mice received aPD-1 therapy. D Double-fluorescent immunohistochemistry of IFN-γ and GSDME-N in heart of WT, Gsdme^Stop/Stop, Gsdme^CR and Gsdme^MR mice received aPD-1 therapy. DAPI was used to stain nuclei. Scale bar, 50 μm. E Representative fluorescent immunohistochemistry staining of CD68 and amplified images in whole heart section of WT, Gsdme^Stop/Stop, Gsdme^CR and Gsdme^MR mice received aPD-1 therapy. The infiltrated CD68⁺ MOs/MPs were obviously noted in the myocardium of WT and Gsdme^CR mice, which were partially inhibited in Gsdme^Stop/Stop and Gsdme^MR mice. Scale bar, 100 μm. F Representative flow cytometry plots and quantitative analysis of Ly6C^hiCD11b⁺ and Ly6C^loCD11b⁺ monocytes in heart of WT, Gsdme^Stop/Stop, Gsdme^CR and Gsdme^MR mice received aPD-1 therapy. G Representative flow cytometry plots and quantitative analysis of CCR2⁻MHC-II^lo, CCR2⁻MHC-II^hi and CCR2⁺MHC-II^hi macrophages in heart of WT, Gsdme^Stop/Stop, Gsdme^CR and Gsdme^MR mice received aPD-1 therapy. The data were presented as means ± SEM and analyzed by One-Way ANOVA followed by Tukey post-hoc test. n = 6 biologically independent experiments.