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. 2024 Jun 28;27(8):110413. doi: 10.1016/j.isci.2024.110413

Figure 6.

Figure 6

The expression of PCDHB4 and in vitro functional analysis in SCLC

(A) qRT-PCR and western blot showing the higher expression of PCDHB4 in SCLC cell lines (H69, H82, H446 and H526) compared with human lung epithelial cells (BEAS-2B).

(B) Representative IHC images of PCDHB4 expression in SCLC clinical specimens. T: Tumor tissues, N: Normal tissues.

(C) PCDHB4 overexpression in SBC2 cells confirmed by qRT-PCR. The differences between groups were analyzed by unpaired t-test (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001).

(D) PCDHB4 overexpression reduces the cell death of SBC2 cells induced by different concentrations of cisplatin. The differences between groups were analyzed by unpaired t-test (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001).

(E) Cell proliferation assay of SBC2 cells transfected with control vector (pCMV-3Tag) and PCDHB4 overexpression vector (pCMV-3Tag-PCDHB4). The differences between groups were analyzed by unpaired t-test (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001).