Protocol name	Step details
AspirateTo4uL Aspirate liquid to approximately 4 μL of residual volume	W- Aspirate Vacuum Filtration: False Travel Rate: 1 CW 4.1 mm/s Delay: 0 msec Z offset: 30 steps (3.1 mm above carrier) X offset: 42 steps (1.92 mm right of center) Y offset: 0 steps (center of well) Secondary Aspirate: Yes Secondary Z offset: 75 steps (9.53 mm above carrier) Secondary X offset: −45 steps (2.06 mm left of center) Secondary Y offset: 0 steps (center of well)
H2O_Prime PBS_Prime	W- Prime Buffer: [A or B] Volume 45 mL Flow Rate: 9 Low Flow Path Volume: 25 mL Submerge tips: No
H2O_Wash_6X160uL PBS_Wash_6X160uL Aspirate liquid to approximately 40 μL, dispense 160 μL of PBS or ddH2O, and repeat for a total of 6 times	W- Wash Pre-dispense before washing: No Bottom Wash: No Number of Wash Cycles: 6 Aspirate per cycle Travel Rate: 1 CW 4.1 mm/s Delay: 0 msec Z offset: 42 steps (5.34 mm above carrier) X offset: 42 steps (1.92 mm right of center) Y offset: 0 steps (center of well) Secondary Aspirate: Yes Secondary Z offset: 80 steps (10.16 mm above carrier) Secondary X offset: −45 steps (2.06 mm left of center) Secondary Y offset: 0 steps (center of well) Dispense per cycle Buffer: [A or B] Volume: 160 μL/well Flow Rate: 1 Z offset: 118 steps (14.99 mm above carrier) X offset: −36 steps (1.65 mm left of center) Y offset: 0 steps (center of well) Pre-dispense: not available Delay start of Vacuum until Volume dispensed: 160 μL/well Shake/Soak after dispense: No Pre-dispense between cycles No Final Aspirate: No
AspirateTo4uL_Peri40uL Aspirate liquid to approximately 4 μL of residual volume and dispense 40 μL of elution buffer using the peristaltic pump with a 5 μL cassette	W- Aspirate Vacuum Filtration: False Travel Rate: 1 CW 4.1 mm/s Delay: 0 msec Z offset: 37 steps (4.7 mm above carrier) X offset: 42 steps (1.92 mm right of center) Y offset: 0 steps (center of well) Secondary Aspirate: Yes Secondary Z offset: 75 steps (9.53 mm above carrier) Secondary X offset: −45 steps (2.06 mm left of center) Secondary Y offset: 0 steps (center of well) P- Dispense Volume: 40 μL/tube Flow Rate: Low Required cassette: 5 μL Secondary Z offset: 336 steps (15.36 mm above carrier) Secondary X offset: −40 steps (1.83 mm left of center) Secondary Y offset: 0 steps (center of well) Pre-dispense: No Columns:111111111111