Table 1.

Recipe for preparation of the XF assay media

Reagent	Final concentration (mM)	Stock concentration (mM)	Stock volume (μL)
Glucose	10 mM	1000	500
Pyruvate	1 mM	100	500
Glutamine	2 mM	200	500

Note: Stock volumes should all be added to 48.5 mL of XF DMEM media to make the final XF assay media (Total volume = 50 mL).