Table 2.

Instructions for preparing stock solutions of oligomycin, FCCP and rotenone/antimycin A in the FCCP optimization protocol

Drug	Final concentration (μM)	Stock volume (μL)	Medium volume (μL)
Port A Oligomycin	1.5	450	2455
	0.125	37.5	2962.5
Port B FCCP	0.25	75	2925
	0.5	150	2850
	1	300	2700
	2	600	2400
Port C Rotenone/antimycin A	0.5	300	2700

Note: Each stock volume should be added to the medium volume. Final volume for each drug will be 3 mL.