Fig. 3.

Liraglutide reduced NETs formation by inhibiting ROS production. The enriched naïve murine neutrophils were incubated with the liraglutide (100 mm) for 1 h, followed by the stimulation with PMA (Sigma‐Aldrich) at 25 nm for 2 h, or incubated with ROS inducer diallyl tetrasulfide. (A) Representative flow cytometry assay of ROS levels and (B) statistical analysis of mean fluorescence intensity (MFI) (n = 3). (C) NETs were detected by Hoechst 33342 and SYTOS Green dye. Scale bar: 400 μm. (D) Quantification of tumor‐derived NETs using a fluorescence microplate reader (n = 6). Nonparametric tests were utilized to assess statistical significance between different treatment groups. *P < 0.05, **P < 0.01, ***P < 0.001. ns, non‐significant. Error bars represent SD. Triple assays were performed.