Fig. 5.
Long‐term immune responses induced by the combination therapy rejected rechallenge. The measurement of IFN‐γ concentration in the culture media of CD8+ T cells from (A, B) lymph node, (C, D) spleen, and (E, F) tumor tissues in LLC or Hepa1‐6 allograft mice after 7 days of treatment. N = 12 mice for every group. (G) LLC or (H) Hepa1‐6 allograft C57BL/6 mice were treated with STR. Mice pre‐treated with PBS, or the combination therapy received tumor rechallenge by administration of respective cell lines. N = 6 mice for every group. Tumor volume = 0.5 × length × width2. Statistical significance was determined with two‐tailed t‐test. Nonparametric tests were utilized to assess statistical significance between different treatment groups. *P < 0.05, **P < 0.01, ***P < 0.001. Error bars represent SD.