Figure 3.

Effect of GPC3 ablation on liver cancer cell growth, migration, and cell invasion. A. Cell growth rate of parental and GPC3-KO cells. Cell proliferation was measured using the BrdU cell proliferation assay kit after 24, 48, 72, and 96 h. The left panel represents HepG2, and the right panel represents Hep3B. The cell growth rate was significantly reduced by GPC3 deficiency in both HepG2 and Hep3B. B. Scratch wound healing assay demonstrates a significant decrease in the rate of wound healing in GPC3-KO cells compared to the wild-type liver cancer cells. C. Cell invasion was significantly inhibited after the knockout of GPC3 expression. Invading cells were assessed by counting cells in five random high-power fields (HPFs). Scale bars are shown for 100 µm. All experiments were performed in triplicate, and error bars represent standard deviations from the mean. Differences in cell growth and migration rates were statistically tested using the t-test. *, P<0.05; ***, P<0.001.