Table 2.
Experimental conditions of Box-Behnken design (Design-Expert 8.0.6 software) for optimization of significant five factors viz. initial pH, temperature (°C), tryptophan concentration (mg/mL), medium strength (g/L) and incubation period (days) with the actual response i.e. Indole acetic acid (μg/mL) produced by GG3F6.
| Run Code | A: Tryptophan | B: Temperature | C: pH | D: Potato dextrose broth | E: Incubation Peroid | R: Indole acetic acid |
|---|---|---|---|---|---|---|
| 1 | 1.00 | 35.00 | 12.00 | 6.00 | 7.00 | 28.128 |
| 2 | 1.00 | 45.00 | 8.00 | 27.00 | 11.00 | 42.5829 |
| 3 | 1.00 | 35.00 | 12.00 | 27.00 | 11.00 | 61.1137 |
| 4 | 2.00 | 35.00 | 8.00 | 48.00 | 7.00 | 105.237 |
| 5 | 1.00 | 35.00 | 8.00 | 27.00 | 7.00 | 43.2938 |
| 6 | 1.00 | 35.00 | 4.00 | 6.00 | 7.00 | 28.8389 |
| 7 | 2.00 | 35.00 | 8.00 | 27.00 | 3.00 | 88.0806 |
| 8 | 2.00 | 45.00 | 8.00 | 27.00 | 7.00 | 113.578 |
| 9 | 0.00 | 35.00 | 8.00 | 27.00 | 3.00 | 36.8957 |
| 10 | 1.00 | 35.00 | 8.00 | 48.00 | 11.00 | 60.4028 |
| 11 | 1.00 | 25.00 | 8.00 | 27.00 | 3.00 | 42.6303 |
| 12 | 1.00 | 35.00 | 12.00 | 27.00 | 3.00 | 62.2512 |
| 13 | 1.00 | 45.00 | 12.00 | 27.00 | 7.00 | 66.6588 |
| 14 | 1.00 | 45.00 | 8.00 | 6.00 | 7.00 | 35.0474 |
| 15 | 1.00 | 45.00 | 8.00 | 48.00 | 7.00 | 66.1374 |
| 16 | 1.00 | 35.00 | 8.00 | 6.00 | 3.00 | 31.6825 |
| 17 | 0.00 | 35.00 | 12.00 | 27.00 | 7.00 | 49.9763 |
| 18 | 0.00 | 35.00 | 8.00 | 48.00 | 7.00 | 49.455 |
| 19 | 1.00 | 35.00 | 12.00 | 48.00 | 7.00 | 121.209 |
| 20 | 1.00 | 35.00 | 4.00 | 48.00 | 7.00 | 55.3318 |
| 21 | 1.00 | 35.00 | 8.00 | 48.00 | 3.00 | 60.2607 |
| 22 | 0.00 | 35.00 | 8.00 | 6.00 | 7.00 | 22.4882 |
| 23 | 1.00 | 25.00 | 8.00 | 6.00 | 7.00 | 41.7773 |
| 24 | 2.00 | 35.00 | 12.00 | 27.00 | 7.00 | 110.924 |
| 25 | 2.00 | 25.00 | 8.00 | 27.00 | 7.00 | 97.3223 |
| 26 | 0.00 | 45.00 | 8.00 | 27.00 | 7.00 | 39.6919 |
| 27 | 1.00 | 35.00 | 8.00 | 27.00 | 7.00 | 44.2417 |
| 28 | 2.00 | 35.00 | 4.00 | 27.00 | 7.00 | 52.2512 |
| 29 | 1.00 | 35.00 | 8.00 | 27.00 | 7.00 | 44.6682 |
| 30 | 1.00 | 35.00 | 8.00 | 27.00 | 7.00 | 46.9431 |
| 31 | 0.00 | 25.00 | 8.00 | 27.00 | 7.00 | 33.9573 |
| 32 | 1.00 | 25.00 | 4.00 | 27.00 | 7.00 | 37.1327 |
| 33 | 1.00 | 25.00 | 12.00 | 27.00 | 7.00 | 64.2891 |
| 34 | 0.00 | 35.00 | 8.00 | 27.00 | 11.00 | 36.327 |
| 35 | 1.00 | 35.00 | 4.00 | 27.00 | 11.00 | 33.7678 |
| 36 | 2.00 | 35.00 | 8.00 | 6.00 | 7.00 | 30.8294 |
| 37 | 1.00 | 35.00 | 8.00 | 6.00 | 11.00 | 27.7962 |
| 38 | 0.00 | 35.00 | 4.00 | 27.00 | 7.00 | 29.1706 |
| 39 | 1.00 | 25.00 | 8.00 | 27.00 | 11.00 | 43.7204 |
| 40 | 1.00 | 45.00 | 4.00 | 27.00 | 7.00 | 36.1374 |
| 41 | 1.00 | 25.00 | 8.00 | 48.00 | 7.00 | 61.5877 |
| 42 | 1.00 | 35.00 | 4.00 | 27.00 | 3.00 | 35.7109 |
| 43 | 1.00 | 45.00 | 8.00 | 27.00 | 3.00 | 39.6919 |
| 44 | 1.00 | 35.00 | 8.00 | 27.00 | 7.00 | 45.9479 |
| 45 | 2.00 | 35.00 | 8.00 | 27.00 | 11.00 | 106.043 |