Fig. 3.

The renal pathological findings of the patient. Electron microscopy revealed segmental hyperplasia of mesangial cells and stroma in the glomerular mesangial area, about 60 % of cases of podocyte peduncle fusion, tubular epithelial cells' vacuolar degeneration (red arrow), a small number of inflammatory cells infiltration in the kidneys' interstitium, and hyperplasia of collagenous fibers (a–c); Immunofluorescence of renal tissues revealed IgG(−) (d); HE staining showed partial glomerulosclerosis (black arrows), the mild proliferation of mesangial cells and stroma in the glomerular mesangial zone, thickening of some glomerular capsule walls, vacuolation of tubular epithelial cells and degeneration of granules, massive bare basement membrane formation, renal tubular epithelial cell cytoplasm and brush border barely visible (green arrow), fragmentation of eosinophilic red in some glomerulonephric tubule lumens (blue arrow), multifocal inflammatory cell infiltration of the renal mesangium (green circle), and moderate-to-severe fibrosis (e-h, 200 × ); Immunohistochemical staining of tubular myoglobin in the lumen of renal tubules was positive, with positive Myoglobin-associated granules indicated by red arrows (i, 400 × ); PAS staining showed renal tubular necrosis (blue arrows) and renal interstitial inflammatory lesions (green arrows) (j, 400 × ); PASM (k, 200 × ) and Masson (l, 200 × ) staining of glomerular sites did not show complexophilic hemoglobin deposition, the red arrows indicate sclerotic glomeruli, the blue circles show atrophied tubules and surrounding dark grey fibrotic areas, the purple granular tubular patterns in the official lumen may be seen in the red circles, and the myoglobin histochemistry suggests positive. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)