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. 1999 Dec;73(12):9702–9709. doi: 10.1128/jvi.73.12.9702-9709.1999

FIG. 2.

FIG. 2

Vectors with hybrid ppET1/LTR promoters. The proviral forms, after transduction, of the vectors ppET1/LTR hybrids 1, 3, and 5 are illustrated, along with the control vector MFGnlslacz (derived from pNeoMFGnlslacz). In the three hybrids, variable amounts of the viral enhancer and promoter within U3 are replaced with ppET1 promoter sequence (solid). Cross-hatched boxes indicate enhancer and R regions of the viral LTR. The NheI, XbaI, and SstI restriction sites used during construction are indicated. Modifications were initially engineered in the 3′ LTR and duplicated at the 5′ LTR during RT. Also indicated are the starts of the expected transcripts, including potential transcripts from the residual viral promoter in hybrids 1 and 3. Average vector titers from four independent experiments, obtained following infection of PAE, 3T3, and TE671 cells and histochemical staining for β-galactosidase, are given as CFU per milliliter. The limit of detection in this assay was 200 CFU/ml for 3T3 and TE671 cells and 20 CFU/ml for PAE cells.

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