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. 1999 Dec;73(12):9702–9709. doi: 10.1128/jvi.73.12.9702-9709.1999

FIG. 4.

FIG. 4

PCR analysis of transduced target PAE and TE671 cells. Cell lysates were used to amplify a ∼500-bp lacZ/vector junction region to detect the presence of integrated proviruses (LacZ). The product is slightly larger for ETP-I due to differences at the cloning site. The lower panels show controls for DNA quantification in the PCR, i.e., human GAPDH or porcine cytochrome oxidase, as appropriate. (A) PAE and TE671 cells were infected with MFGnlslacz control (lane C) or ETP/LTR hybrid 1, 3, and 5 viruses. Lane − contains uninfected controls. (B) PAE cells were infected with MFGnlslacz, ETP-I, and ETP/LTR hybrid viruses, diluted as shown. As is evident from the dilution series for MFGnlslacz, transduction is initially nonlinear, and so quantitation should be estimated from the 1:10-diluted viruses.

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