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. 1999 Dec;73(12):9702–9709. doi: 10.1128/jvi.73.12.9702-9709.1999

FIG. 6.

FIG. 6

Endothelial cell specificity of expression from ETP/LTR hybrid 5. PAE, 3T3, and a panel of six human cell lines, including primary endothelial cells (Primary EC), were infected with virus ELH5.10 for titer determination by histochemical staining or for determination of β-galactosidase enzyme activities. The MFGnlslacz control virus was used in parallel. Three independent virus harvests of ELH5.10 were used. For each target cell, the titer of ELH5.10 relative to the control (A) or the β-galactosidase activity normalized to account for the variation in intrinsic infectability of the different target cells (B) is shown. (A) The relative titres are expressed as a percentage of that displayed on PAE cells in order to normalize variations in the absolute titers of the different harvests. Data shown are the mean and standard error of nine determinations. (B) Levels of expression per transduced cell for each cell target following infection with ELH5.10 (but see the text for a discussion of absolute values). Data shown are the mean and standard error of six (HT1080, EAhy926, and HMEC-1 cells) or nine (PAE, TE671, and 3T3 cells) determinations. The mean activity of the control virus in PAE cells was 0.8 pg/cell. Control virus titers were of the order of 107 CFU/ml for TE671 and 3T3 cells, 106 CFU/ml for HT1080, EAhy926, and PAE cells, 105 CFU/ml for ECV304 and HMEC-1 cells, and 104 CFU/ml for primary endothelial cells.

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