TABLE 1.
Artificial joints formed in vitro during inverse nested PCR
Source of cell DNA | Cell DNA (pg)a | DHBV3 DNA (pg)a | No. of reactions | DHBV16 productsb | DHBV16 artifactsc | DHBV3 productsc | DHBV3 artifactsc |
---|---|---|---|---|---|---|---|
Uninfected liver | 200 | 0.06 | 30 | 0 | 0 | 15 | 15 |
Infected liver | 50 | 0.015 | 96 | 4 | 1 | 22 | 22 |
Amount of cellular or plasmid DNA per reaction.
Total products sequenced that contained DHBV16 or DHBV3 DNA.
Joints due to ligation in vitro; see the text for detailed explanation.