FIG. 1.

Construction of ICP0/VP16 double mutants KM100 and KM110. (A) Schematic diagram of the VP16 and ICP0 loci, indicating the locations of the linkers corresponding to the in1814, V422, and n212 mutations. The C-terminal acidic activation domain of VP16 is displayed as a shaded box. Numbers refer to amino acid residues. Diagrams are not to scale. (B) Southern blot analysis of viral DNA from wild-type and mutant viruses. Viral DNA was digested with the indicated restriction endonucleases and subjected to Southern blot hybridization as described in Materials and Methods.