FIG. 5.
Detection of a 40-kDa protein in cells transfected with LR gene constructs. Human (293) cells were transfected with the designated plasmids, whole-cell extract was prepared 48 h after transfection, and 50 μg of protein was electrophoresed on an SDS–12% polyacrylamide gel. Extracts were prepared from mock-transfected cells (A) and cells transfected with LRTwt (B), LRTΔHX (C), LRTstop (D), and LRTΔSmaI (E). Locations of molecular weight markers are shown at the left in kilodaltons. The arrow indicates the position of LRP.