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PCR settings for the primer pairs used
| Target gene | Primer pair | Initial denaturation | Denaturation | Annealing | Elongation | Final elongation | No. of cycles |
|---|---|---|---|---|---|---|---|
| “Universal” (16S rRNA gene) | 515FB - 806RB | 3′, 94°C | 45″, 94°C | 1′, 50°C | 1′ 30″, 72°C | 10′, 72°C | 35 |
| Archaea (16S rRNA gene) | 344F-1041R | 5′, 95°C | 30″, 94°C | 45″, 56°C | 1′, 72°C | 10′, 72°C | 25 |
| 519F-806R | 5′, 95°C | 40″, 95°C | 2′, 63°C | 1′,72°C | 10′, 72°C | 30 | |
| Fungi (ITS region) | ITS86F - ITS4 | 1′, 94°C | 30″, 94°C | 30″, 56°C | 30″, 68°C | 10′, 68°C | 35 |
