Fig. 3.

Effect of Abi on mitochondria respiration. After treatment with Abi for 72 h, T98G A and U87MG-R B cells were subjected to Seahorse XF Cell Mito Stress Test. Experiments, each containing two replicates, were performed independently three times. C Upper: Primary and recurrent specimens from GBM patients were homogenized and subjected to RNA-seq. Genes related to Complex I to V in mitochondria were shown in the heatmaps. Lower: RNA extracts from Pt#3 and Pt#3-R were prepared for RT-qPCR. D After treatment with Abi for 72 h, U87MG-R and T98G E cells were harvested for RNA extraction, followed by RT-qPCR. Experiments, each containing two replicates, were performed independently three times. (*P < 0.05, **P < 0.01, ***P < 0.001)