Fig. 4.

Abi suppresses mitochondria respiration through decreasing CDK1 expression. After treatment with Abi for 72 h, RNA extracts of T98G cells were subjected to RNA-seq. Genes influenced by Abi were shown A and functional clustered by Gene Set Enrichment Analysis (GSEA) B. C Abi-downregulated genes which were involved in G2/M transition and DNA replication were shown. D Western blotting for cyclin A2 and CDK1. E After treatment for 48 h, T98G cells were treated with 20 µM CHX for the indicated time interval, and cell lysates were collected for Western blotting. F Left: After transfection for 24 h, T98G cells were fixed and immunostained using the anti-GFP antibody. Right: After transfection for 24 h, cells were treated with Abi with or without Prog for 72 h. Cell viability was estimated using the CCK8 assay. Experiments, each containing three replicates, were performed independently three times. IC50 was calculated using the Prism software. G After transfection for 24 f and treatment with Abi for the additional 72 h, mitochondria respiration in T98G cells was estimated using Seahorse XF analyser. Experiments, each containing two replicates, were performed independently three times