FIG. 2.

Autoradiogram of RT-PCR amplicons generated by using total RNA from PrV-Be-infected cells. The identity of the cell line is indicated above each blot. The products were generated by PCR with primers LLT3.1 and CM3.1 and cDNA synthesized from the total RNA extracted from cells at 0 to 24 h p.i. (as indicated above each lane) with primer LAT-R as templates. The resultant amplicons were resolved in an agarose gel, transferred to a nylon membrane, and then hybridized with ³²P-labeled DNA probe I. The positive control (P) is the result of PCR amplification of pAC38 using primers CM3.1 and LLT3.1. The expected size of the amplicon arising from a spliced LAT-like transcript is shown on the right.