FIG. 3.

Electrophoregram and autoradiogram of RT-PCR products derived from total (lanes T) and poly(A) (lanes A) RNAs isolated from PrV-infected N1E and MDBK cells at 6 h p.i. Products were generated by using cDNAs synthesized from RNAs with primer R2280. Primer pairs LLT3.1-CM3.1 and R1669-L808 were used for the specific amplification of a spliced region analogous to that of the LLT. The products were resolved in an 1.5% agarose gel (A), transferred to a nylon membrane, and hybridized with ³²P-labeled DNA probe I (B). The anticipated amplicon sizes are shown on the right.