FIG. 4.

Sequence alignment of the in vitro PrV LAT RT-PCR product with the reported in vivo cDNA sequence of the PrV LLT (GenBank accession no. M57505 [9]). (A) The sequence sources (in vivo LLT cDNA and the 208-bp RT-PCR products from the corresponding PrV-infected cells) are shown on the left. (B) LAT, the cDNA sequence of the LLT expressed during latency; N1E, the sequence of the 831-bp RT PCR products generated by using poly(A) RNA from PrV-infected N1E cells at 6 h p.i. with primers R2280, R1669, and L808. Dashes, identity to the published sequence; underlined nucleotides, transversions in the PrV-Be strain genome. The juncture between the first and second exons of the processed LLT is between the boldface nucleotides.