FIG. 5.

Northern hybridization of total and poly(A) RNAs from PrV-infected cells. (A) Polarity and relationship of probes and transcripts from the LAT gene region during latency (in vivo) and productive infection of cultured cell lines. The arrow above transcripts represents the direction of transcription of the LAT gene. (B) Results of Northern hybridization of total (lanes T) and poly(A) (lanes A) RNAs from PrV-infected neuronal cells at 6 h p.i. RNAs were separated in a 2.2 M formaldehyde–1% agarose gel and then transferred to a nylon membrane. The blot was sequentially hybridized with ³²P-labeled ssRNA probes Ic, IIc, IVc1, IIIc, and IVc2. Molecular size markers are indicated on the right. (C) At 6 h p.i., RNAs were isolated from PK-15 (lanes P), CRFK (lanes C), and MDBK (lanes M) cells. The RNAs [total (lanes T) and poly(A) (lanes A)] were separated in a 2.2 M formaldehyde–1% agarose gel and then transferred to a nylon membrane. The blot was hybridized with ³²P-labeled ssRNA probe Ic. Molecular size markers are indicated on the right.