Northern hybridization of total and poly(A) RNAs from PrV-infected cells. RNAs from PK-15 (lanes P), MDBK (lanes M), CRFK (lanes C), and N1E (lanes N) cells were harvested at 6 h p.i., separated in a 2.2 M formaldehyde–1% agarose gel, and then transferred to a nylon membrane. RNAs were hybridized with 32P-labeled ssRNA probe IIa, which is complementary to the EP0 mRNA and has the same polarity as LAT. Molecular size for the EP0 mRNA is indicated on the right.