FIG. 7.

Autoradiogram and sequence analysis of 5′ RACE product generated by using RNAs from PrV-infected N1E and MDBK cells. The cDNA for 5′ RACE analysis was generated by using total RNA (MDBK [MT] and N1E [NT]) or poly(A) RNA (MDBK [MA] and N1E [NA]) from infected cells 6 h p.i. with primer R998 (for MT and NT) or LAT-R (for MA and NA). (A) Autoradiogram of 5′ RACE products amplified only with primers R832 and AAP (lanes 1 to 4) or subsequently with primers R334 and AUAP (lanes 5 to 8) and then hybridized to DNA probe II. (B) Sequence alignment of the 100-bp 5′ RACE product with the reported cDNA sequence of the PrV LLT (GenBank accession no. M57505 [9]). 5′ RACE, product generated by using total RNA from infected N1E cells with primers AUAP and R334; LAT, published cDNA sequence of the LLT. Nucleotides in lowercase are insertions that occurred in the PrV-Be genome. The underlined nucleotides represent the genetic differences between PrV-Be and the published in vivo PrV-Funkhauser LLT cDNA sequence.