FIG. 8.

Autoradiograms of the LAT RT PCR amplicons generated by using total RNA from PrV-infected cells treated with cycloheximide or araC. Virus-infected MDBK and N1E cells were treated with either cycloheximide (A) or araC (B) prior to total RNA extraction at 0 to 24 h p.i. (as indicated above each blot). RT-PCR products were synthesized from total RNA by using primers LAT-R (RT) and CM3.1 plus LLT3.1 (PCR). The products were resolved in an 1.5% agarose gel, transferred to a nylon membrane, and then hybridized with ³²P-labeled DNA probe I. The positive control (P) is the PCR product obtained with pAC38 and primers CM3.1 and LLT3.1. The expected size of the amplicon arising from a spliced LAT-like transcript is shown on the right.