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. Author manuscript; available in PMC: 2024 Aug 6.
Published in final edited form as: Oncogene. 2017 Apr 24;36(35):4953–4962. doi: 10.1038/onc.2017.111

Figure 4.

Figure 4.

Bim deficiency protects cells from immunotoxin-mediated cell death. Caspase 3/7 activity and cell viability were determined in KB3-1 WT, empty vector, Bim KO and Bid KO cells after 16 or 24 h of treatment with immunotoxin at 10 ng/ml. Caspase 3/7 activity was determined using the Caspase-Glo3/7 kit. The luminescence of each well was measured and the values are presented as a fold increase relative to untreated cells. Viability was determined using the CellTiter-Glo Luminescent Assay kit. The luminescence of each well was measured and the values are presented as a percentage relative to untreated cells. Data are from at least three independent experiments in triplicate. Error bars display s.d. value. Two tailed unpaired t-tests were performed with ***P < 0.001.