Figure 6.

Response of hematological cell lines to an immunotoxin directed to CD22. (a) Caspase 3/7 activity and cell viability were determined in Nalm6, Raji and CA46 cells after 16 or 24 h treated with immunotoxin in a dose-dependent manner. Caspase 3/7 activity was determined using the Caspase-Glo3/7 kit. The luminescence of each well was measured and the values are presented as a fold increase relative to untreated cells. Viability was determined using the CellTiter-Glo Luminescent Assay kit. The luminescence of each well was measured and the values are presented as a percentage relative to untreated cells. Data are from three independent experiments in triplicate. Error bars display s.d. value. (b) The expression level of various Bcl-2 family proteins was determined from lysates of each cell line, as indicated, by western blot. Blots are representative of two experiments. (c) CA46, Nalm6 and Raji cells were exposed for 16 h to immunotoxin in a concentration-dependent manner. The levels of Bim and Mcl-1 from lysates were visualized by western blot as indicated. Blots are representative of three experiments.