Figure 5.

Puroswitch incorporation into newly synthesized proteins of rat primary neurons. A) Microscopic immunocytochemistry images of primary neurons stained with anti-MAP2 antibodies (cyan), DAPI (blue), and anti-puromycin antibodies (red). Neurons were incubated with or without puroswitch (5 μM) for 1 hour with 390 nm irradiation (100 ms every 10s) or in the dark. Cycloheximide (CHX) was also added as an additional control. B) Relative puroswitch incorporation for different compound treatments. Error bars represent mean values ± SEM. Two-way ANOVA with Tukey’s multiple comparisons were performed (****, p<0.0001; ***, p<0.001).