Figure 3.
The E(z) and jing knockdowns had increased plasticity of melanization in the forewings compared to the ampr dsRNA-injected adults. (a) Forewings of ampr, Pc, E(z) and jing dsRNA-injected O. fasciatus at different temperatures. The arrowhead indicates the melanin in the proximal band blending with the melanized membranous portion of the forewing. (b) Diagram showing the area measured to determine the amount of melanization of the wings. The proportion was calculated by measuring the total melanized area and dividing it by the total area of the forewing. (c) E(z) and jing knockdown wings had increased plasticity in the forewings as a function of temperature. (d) Normalized amount of forewing melanization. The amount of melanization for each treatment was divided by the average amount of melanization for the respective knockdowns at 26.5°C. Results of the one-way ANOVA with Tukey HSD conducted for values at 20 and 33°C are represented by the letters where distinct letters indicate statistically significant differences.